Programs of analyses

Hardware&software MECOS-C2 system can be used for different types of biospecimen analyses, as investigational as special medical standards restricted.

Below ^м index denotes techniques for medical purpose, ^I index denotes techniques of investigational purpose only.

Programs for hematology

Programs for helminth, urine sediment, spermatozoa, bacterial clumps analyses

Multipurpose programs

Programs for hematology

Common properties

Efficient ergonomics, routine operations automation, visual control give advantages in exactness above manual microscopy.

Visual control by a doctor of automated cell sorting (galleries on a computer monitor) makes possible  to correct quickly galleries compositions with sorting exactness increase up to 100%.

Compact viewing of cells in galleries increases pathological morphology detection. Integration of flow hemoanalyzer data base is available.

Analyses norm diapason depending of patient age is tuned automatically.

Statistics gathering for local norm region definition is available. 

Analysis results in standard form can be printed and fixed in database.

Database viewing allows to realize retrospective quality control of specimen preparation and staff mistakes in analyses results.

Forming of special user menu of cell sorting is available.

The programs realize quality control of blood smear, using measurements of erythrocytes staining and layer thinness in sample gathering region.

Complete quality control of automated functions including visualization of smear screening trajectories and cell detection in special reference virtual slide is available. 

Staff teaching using reference virtual slides is available. Statistics can be gathered for external quality control.

Telepathology, remote consultations are available.

Smear preparation by spatula  or by centrifuge are possible. 

Options recommended: smear preparation and staining devices.  

The ERYTHROCYTE ANALYSIS (MECOS-ERA^м) technique for complete set with manual or motorized microscope performs dialogue microscopy of stained blood smear. It makes a quantitative evaluation of anysocytosis and poikilocytosis, builds a Price-Jones graph, distribution of the hemoglobin quantity within the erythrocytes, distribution of erythrocytes by the shape, the 8 types formula of the pathological erythrocytes (schizocytes, megalocytes, echinocytes, stomatocytes, elliptocytes, teardrop cells, target cells, bite cells), erythrocyte ellipse coefficient.

The BLOOD SMEAR ANALYSIS (MECOS-BSA^м) program for motorized complete set performs automatic microscopy of a stained blood smear. BSA scans smear by adaptable path and forms samples of leukocytes and erythrocytes of a given volumes. BSA performs a quantitative analyses of erythrocytes as MECOS-ERA technique and calculates the 12 types differential formula of leukocytes (segmented neutrophyles, lymphocytes, monocytes, eosinophyles, basophyles, band neutrophyles, blast cells, promyeloblasts, myeloblasts, metamyelocytes, lymphocyte variant forms, plasma cells). Under 5% of relocations by a doctor of normal type leukocytes between  galleries usually needed with simply pressing a key. For more details see MECOS-C2 introduces quality standard of blood smear analyses (pdf 3Mb)

The ANIMAL BLOOD SMEAR ANALYSIS (MECOS-ABSA^м) program for motorized complete set is a version of MECOS-BSA for animal blood smear analysis (rat, mouse, dog, cat, other animals by special order).

The HEMO ANALYSIS (MECOS-HEMO^м) program for motorized complete set integrates tools of MECOS-BSA and MECOS-FDMM, performs additionally large leukocyte sample gathering (up to 5000 cells), given type leukocyte sample gathering. Lymphocytogram, monocytogram, neutrophilogram can be calculated.

The RETICULOCYTE ANALYSIS (MECOS-RETA^м) program for complete set with manual or motorized microscope counts concentration and distribution of reticulocytes in blood smear. If motorized, the technique inputs needed number of field of view in thin part of smear and reticulocyte recognition is performed at multiple focus positions. Automatically detected reticulocytes and erythrocytes are marked. Indexes of Reticulocyte Production (RPI) and Immature Reticulocyte Fraction (IRF ) are calculated.

The PLATELET ANALYSIS (MECOS-PLA^м) program for complete set with manual or motorized microscope is analogue of MECOS-RETA and is intended for platelet concentration and distribution analysis. Automatically detected platelets and erythrocytes are marked.

The MYELOGRAM FORMING (MECOS-MYF^м) technique for complete set with manual or motorized microscope provides with special menus and blanks of standard myelogram forming. The program has embedded marrow cells atlas.

The FLOW HEMOANALYZER INTEGRATION (MECOS-FHI^м) program for motorized MECOS AMS allows to integrate data of MECOS-BSA and flow hemoanalyzer.

Programs for helminth, urine sediment, spermatozoa, bacterial clumps analyses

The HELMINTH AND PROTOZOA ANALYSIS (MECOS-HPA^м) technique for complete set with manual or motorized microscope is integration of MECOS-VA and special menus and blanks of standard helminth and protozoa analysis. The program has embedded helminth and protozoa atlas. Ether-formalin precipitation together with PARASEP test-tubes and FE-2 workstation (DiaSys Corporation) specimen preparation is recommended.

Options recommended: specimen preparation (test tubes, workstation).

The HELMINTH DETECTION (MECOS-HPD^м) technique for motorized complete set performs automatic screening of a water, excrement or washout specimen with detection of helminth eggs and forming of detected objects gallery for viewing and dialogue classification. Ether-formalin precipitation together with PARASEP concentrator tubes and FE-5 workstation (DiaSys Corporation) specimen preparation is needed. For more details see MECOS-C2 introduces quality standard of faecal parasite analyses (pdf 1Mb)

The URINE SEDIMENT ANALYSIS (MECOS-USA^м) program for motorized complete set is integration of MECOS-VIM and special menus and blanks of standard urine sediment analysis. The program has embedded urine sediment atlas.

Options recommended: specimen preparation centrifuge.

The SPERMATOZOA MOBILITY AND MORPHOLOGY ANALYSIS technique (MECOS-SMA^I) for complete set with manual or motorized microscope:

processing of video with recording of microscope field of view during 2-10 sec under objective 20x phase contrast, above 0.4 M pixel&30 fps video resolution;

Dialogue choice of episode of best spermatozoa motion analysis;

Full number of spermatozoa measurement;

Spermatozoa mobility parameters measurements:

VAP - Average path velocity, mm/s;

VSL  - Straight-line velocity, mm/s;

VCL  - Curvilinear velocity, mm/s;

ALH  - Amplitude of lateral head displacement, mm;

BCF  - Beat-cross frequency;

STR  - Straightness VSL/VAP;

LIN  - Linearity VSL/VCL.

4 mobility type spermatozoa sorting (according WHO).

Morphology analysis of colored sperm:

automated or dialogue detection of cells; dialogue morphological sorting.

The BACTERIAL CLUMPS ANALYSIS (MECOS-BCA^I) technique for macro viewing counts up automatically number of bacterial clumps in Petri dish. The technique works satisfactorily even clumps have different sizes and partially intersected. Every clump is marked by red dot and user can control and correct result of counting.

Multipurpose programs

The FLUDENSIMORPHOMETRY (MECOS-FDMM^I) program for complete set with manual or motorized microscope provides means of quantitative analysis of cell and other object morphology, optical density and fluorescent markers within the specimens of different types. There are means of: cell objects boundary highlighting (automated of contrast and semi-automated of poor-contrast boundaries); measurement of more than 20 cell parameters, including optical density, geometrical and textural characteristics, mutual location, angles, fragment and hole quantities, max and min diameters, nucleus/cytoplasm ratio, object field of view concentration. Color fluorescent markers can be consisted with transmitted light images and their concentrations inside segmented objects can be calculated. Other markers such as AgNOR, HER2/neu, Ki-67 also can be segmented and measured.

The FLUDENSIMORPHOMETRY and DIAGNOSTICS (MECOS-FDMMD^I) program in addition to tools of MECOS-FDMM can form quantitative diagnostic (differential) decision rules on the base of measurements of object population of a teaching specimen samples. Educable neuron nets are used for rules forming.

The MOBILITY ANALYSIS (MECOS-MA^I) program for complete set with manual or motorized microscope provides with means of shooting a real-time movie about mobile objects. Parameters of mobility and space distribution are calculated.

The SCREENING and MOBILITY ANALYSIS (MECOS-SMA^I) program for motorized MECOS AMS provides with means of shooting a real-time movie about mobile objects. Shooting can be done automatically in selected points along automatically formed screening path. Parameters of mobility and space distribution are calculated.

The TELEMICROSCOPY (MECOS-TEM^м) program for motorized complete set provides with means of on-line remote microscope control. Remote expert can see current field of microscope view on his PC screen and can change objectives, relocate and focus (autofocus, multifocus) specimen. Expert can fix and measure objects of a field of view, form and view trajectory of screening. MECOS-C2 and expert must be linked by fast line (from 100 Kb/s). 

The SPHEROMETRY (MECOS-SPH^I) for complete set with manual or motorized microscope realizes automated segmentation and measurement of touching roundish granules forming statistics of diameters. Measurement error is under 2%.